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Molex Connector Part Number –
Blotting Membrane and Paper: Biotinylated Protein Ladder Detection Pack: Prepare solutions with reverse osmosis deionized RODI or equivalently purified water. It should be noted that for the best possible results a fresh blot is always recommended. Dattasheet answers on our FAQs page.
From sample preparation to detection, the reagents you need for your Western Blot are now in one convenient kit: Electrotransfer to nitrocellulose membrane datashfet Aspirate media from cultures; wash cells with 1X PBS; aspirate. More about how we get our images. Application Dilutions Western Blotting 1: FXR2 Antibody – Detection of Proteins Directions for Use: Do not aliquot the antibody. Incubate substrate with membrane for 1 minute, remove excess solution 5111 remains wetwrap in plastic and expose to X-ray film.
Prepare solutions with reverse osmosis deionized RODI or equivalent grade water. Each of the fragile X proteins can self-associate, as well as form heteromers with the other two related proteins 3.
This can be done by re-exposing the blot to ECL reagents and making sure there is no signal prior to adding the next primary antibody. Sonicate for 10—15 sec to complete cell lysis and shear DNA to reduce sample viscosity.
Microcentrifuge for 5 min. Reprobing can be a valuable method but with each reprobing of a blot there is potential for increased background signal. Proceed with detection Section D.
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Treat cells by adding fresh media containing regulator for desired time. Protein Blotting A general protocol for sample preparation.
Changing to another country might result in loss of shopping cart. Immediately scrape the cells off the plate and transfer the extract to a microcentrifuge tube. Would you like to visit your country specific website? daasheet
Primary Antibody Dilution Buffer: Antibodies are purified by protein A and peptide affinity chromatography. Solutions and Reagents From sample preparation to detection, the reagents you need for your Western Blot are now in one convenient kit: Volumes are for 10 cm x 10 cm cm 2 of membrane; for different sized membranes, adjust volumes accordingly.
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dtaasheet Wash three times for 5 min each with 15 ml of TBST. Reprobing of an existing membrane is a convenient means to immunoblot for multiple proteins independently when only a limited amount of sample is available. To Purchase S View sizes. Incubate membrane in 25 ml of blocking buffer for 1 hr at room temperature.
Dilute to 1X with dH 2 O. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human FXR2 protein.
Additionally, it is recommended that you verify the removal of the first antibody complex prior to reprobing so that signal attributed to binding of the new antibody is not leftover signal from the first immunoblotting experiment.
Please refer to primary antibody datasheet or product webpage for recommended antibody dilution.